Makassar Pharmaceutical Science Journal (MPSJ)

ANALISIS KANDUNGAN FORMALIN PADA IKAN KERING DI PASAR TANJONGE SENGKANG KABUPATEN WAJO

Zainal Abidin — 2025-04-30

Formalin (formaldehyde) is one of the chemical compounds banned by BPOM as a preservative in food additives. This study aims to determine the content of formalin (formaldehyde) in dried fish circulating in the Tanjonge Sengkang market, Wajo Regency. The sampling method used was purposive side and the test method used was qualitative analysis method using KMnO4 reagent, Tollens, Nash, Fehling A and Fehling B and Chromic Acid. The test results using the KMnO4, Tollens, Nash, Fehling A and Fehling B reagents and Chromatophytic Acid of the nine dried fish samples did not contain formalin.

Uji Aktivitas Antioksidan Minyak Nilam (Pogostemon cablin Benth.) Dengan Metode FRAP

Nabila Ikhwanun Niza — 2025-04-30

ABSTRACT

Patchouli oil (Pogostemon cablin Benth.) is an essential oil derived from the leaves of the patchouli plant. This oil is recognized for its primary component, patchouli alcohol, an antioxidant. The antioxidant properties of patchouli oil effectively inhibit free radical oxidation through the exchange of hydrogen atoms, forming more stable compounds. This study seeks to evaluate the antioxidant potential of patchouli oil through the FRAP method, utilizing a UV-Vis spectrophotometer for measurement at a wavelength of 692 nm. The findings indicate a regression equation of y = 0.0258x + 0.0256, with correlation coefficient of r = 0.9981 and an antioxidant activity measured at 1.7831 mgQE/g extract.

Keywords: Antioxidant; Patchouli oil (Pogostemon cablin Benth.); FRAP method; UV-Vis spectrophotometer

ABSTRAK

Minyak nilam (Pogostemon cablin Benth.) merupakan minyak atsiri yang diperoleh dari daun tanaman nilam. Minyak ini dikenal memiliki kandungan utama patchouli alcohol, yang berfungsi sebagai antioksidan. Aktivitas antioksidan minyak nilam dapat menghambat oksidasi radikal bebas dengan cara menyumbangkan atom hidrogen, sehingga menghasilkan senyawa yang lebih stabil. Penelitian ini bertujuan untuk mengetahui potensi minyak nilam sebagai antioksidan menggunakan metode FRAP, kemudian diukur menggunakan spektrofotmeter UV-Vis pada panjang gelombang 692 nm. Dari penelitian ini diperoleh persamaan regresi y = 0,0258x + 0,0256, r = 0,9981 dan aktivitas antioksidan sebesar 1,7831 mgQE/g ekstrak.

Kata kunci: Antioksidan; Minyak nilam (Pogostemon cablin Benth.); Metode FRAP; Spektrofotometer UV-Vis

PENGUJIAN AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL BUNGA KERSEN (Muntingia calabura L.) MENGGUNAKAN METODE FRAP

Siti Sumayya Kaisupy — 2025-04-30

Kersen Flower (Muntingia calabura L.) contains several chemical compounds, including flavonoids, tannins, triterpene, saponins and polyphenols that play a role in antioxidant activity. This study aims to determine the antioxidant activity and determine the antioxidant activity value of ethanol extract Muntingia Flower (Muntingia calabura L.) using FRAP (Ferry Reducing Antioxidant Power) method. Extraction was done using maceration method and measurement was done using UV-Vis spectrophotometry method at wavelength of 715 nm. The comparator used was quercetin. The results showed that the concentration of antioxidant activity Muntingia Flower using FRAP method was 684.209 mgQE/g which showed high activity.

GAMBARAN MAKROSKOPIK LIMPA TIKUS (Rattus norvegicus) PADA PEMBERIAAN EKSTRAK ETANOL DAUN GINSENG (Talinum paniculatum (Jacq.) Gaertn) YANG DIINDUKSI OVALBUMIN

Multazania Multazania — 2025-04-30

Fameflower Leaves (Talinum paniculatum (Jacq) Gaertn) are medicinal plants that communities have traditionally used due to their active compounds, such as saponins, flavonoids, tannins, triterpenes, sterols, and polyphenols found in both the leaves and roots. This study aimed to determine the dose of ethanol extract of fameflower leaves affecting the histology of the spleen in male rats (Rattus norvegicus) following ovalbumin antigen stimulation, as well as to observe changes in the spleen tissue structure after the extract treatment. The study used 20 Wistar rats divided into four groups. The rats were first adapted for 2 weeks and then fasted for 8 hours before the treatment—a group I served as the standard control. Group II was induced with ovalbumin antigen. Groups III and IV were induced with the antigen, and the ethanol extract of fameflower leaves was administered at doses of 0.4g/kgBW and 0.8g/kgBW, respectively. The extract was administered orally for 7 days to the test animals. On day 8, the test animals were sacrificed, and their spleens were harvested for histological preparation using the Hematoxylin-Eosin staining method. Microscopic observation showed no histological damage in the spleen, such as inflammation, degeneration, congestion, hemorrhage, or necrosis. Therefore, it can be concluded that the ethanol extract of Bugis Ginseng leaves did not affect the spleen of rats at doses of 0.4g/kgBW and 0.8g/kgBW

STUDI ETNOFARMASI TUMBUHAN OBAT SEBAGAI ANTIHIPERTENSI DI DESA PATTALLASSANG, KECAMATAN PATTALLASSANG KABUPATEN GOWA

Putri Nur Fadhilah — 2025-04-30

Hypertension remains a prevalent global health concern and leading cause of mortality worldwide. In Pattallassang Village, traditional medicine utilizing medicinal plants persists as a common therapeutic approach for hypertension management. This study aimed to systematically identify, document and evaluate the ethnomedicinal knowledge of local communities regarding antihypertensive plants. Employing a descriptive observational design, data were collected through structured interviews and questionnaires administered to 100 respondents. The investigation identified 31 plant species utilized as antihypertensive agents, with the most prevalent being Allium sativum (garlic), Cucumis sativus (cucumber), Annona muricata (soursop leaves), Apium graveolens (celery), and Syzygium polyanthum (bay leaf). The leaves were boiled and the decoction was consumed as herbal infusions. Quantitative analysis using Use Value (UV) indices revealed Allium sativum as the most utilized species (UV=0.41), followed by Cucumis sativus (0.26), Apium graveolens (0.16), and Annona muricata (0.15). These findings demonstrate the preservation of significant ethnobotanical knowledge within the community regarding plant-based hypertension treatment. The study highlights the need for: (1) conservation efforts to protect this traditional knowledge, and (2) further pharmacological research to validate the therapeutic efficacy of these plants.

Keywords: Ethnopharmaceutical; medicinal plants; Hypertension; Traditional Medicine; Pattallassang Village