STUDI KOMPARASI AKTIVITAS ANTIRADIKAL BEBAS ANTARA EKSTRAK METANOL DAUN DAN KULIT KAYU TUMBUHAN KEDONDONG PAGAR (Lannea coromandelica (Houtt.) Merr) DENGAN METODE PEREDAMAN SENYAWA 1,1-DIPHENYL -2-PICRYLHYDRAZIL (DPPH)
Keywords:
Free anti-radical activity, Methanol extract, Lannea coromandelica(Houtt.) Merr, UV-Ms Spectrophotometry, DPPH
Abstract
Lannea coromandelica (Houtt.) Merr is one of the plants that hast he potential to have free antiradical activity. The research aimed to compare the free antiradical activity of methanol extract of the bark and leaves of Lannea coromandelica (Houtt.) Merr using the free radical reduction method 1.1 diphenhyl-2-picrylhydrazil (DPPH). The bark nd the leaves extract of the tree were obtained by maceration using methanol. The methanol extract preliminary tests were conducted by TLC using n-hexane: ethyl acetate (9 : 1) and ( 7: 3). The positive results for free antiradical aoctivity marked with purple to yellow spots after DPPH spraying was obtained. The thick bark and leaves extract were then quantitatively tested for free antiradical activity to obtain lC50 values using spectrophotometry at the maximum wavelength of 516 nm. The results of data analysis showed that methanol extract of its bark and leaves had lC50 of 20.778 µg / mL and 19.819 µg / mL, with ascorbic acid as a comparison having an lC50 value of 6.816 µg / mL. So it can be concluded that the methanol extract of the leaves of"Lannea coromandelica (Houtt) Merr has stronger anti-radical activity than its bark.
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Badarinath, A, V, K, M, Rao, C, M, M, S, Chetty, S, Ramkanth, T, V, S, Rajan and K, Gnanaprakash 2010, ‘ A review om In-vitro Antioxidant Methods: Comparasions, Correlations and Considerations, International Journal of Pharmaceuticsn Technology Research , 2 (2) : m1276-1285.
Ditjen POM 2000, ‘Parameter Standar Umum Ekstrak Tumbuhan Obat’, Cetakan Pertama, Jakarta: Departemen Kesehatan RI, Jakarta.
Erviana, L, Malik, A, & Najib, A 2016, ‘Uji Aktivitas Antiradikal Bebas Eksrak Etanol Daun Kemangi (Ocimum basilicum L.) Dengan Menggunakan Metode DPPH, Jurnal Fitofarmaka
of the Antioxidant, Antimicrobial and Thrombolytic Activity of the Bark and Leaves of lannea coromandelica (Anacardiaceae), ‘International Journal of Pharmaceutical Sciences and Research, Vol, 4(7): 2609-2614.E-ISSN: 0975-8232; P-ISSN: 2320-5148.
Molyneux, P 2004, ‘The Use of the Stabel Free Radical Diphenylpicryl Hydrazyl (DPPH) for Estimating Antioxidant Activity’, Songklanakarin Journal Science and Technology, Medicine, Surabaya.
Pakaya, D, 2014 ‘Peranan Vitamin C dalam Kulit’, Jurnal Kimia Kedokteran, Vol.1, No,2
Phongpaichit, S, Nikom, J, Rungjindamai, N, Sakayaroj, J, Hutadilok-Towatana, N, Rukachaisirikul, V, & Kirtikara, K, 2007, ‘Biological activities of extracts from endophytic fungi isolated from Garcinia plants’,FEMS Immunology & Medical Microbiology, vol 51, no, 3, hal. 517-525.
Handayani, V, Ahmad, R,A, Sudir, M 2014, ‘Uji Aktivitas Antiokidan Ekstrak Metanol Bunga dan Daun Patikala (Etlingera elatior (Jack) R.M.Sm) Menggunakan Metode DPPH, Pharm Sci Res, Vol. 1, No. 2, pp. 86-93.
Intan, AC 2007, ‘Uji Aktivitas Antioksidan dari Ekstrak Kulit Batang Kedondong pagar (Lannea coromandelica) dengan Metode DPPH (2,2-difenil-1-pikrilhidrazil)’,
Ivanišová, et al 2013, ‘Antioxidant Activity of Selected Plant Products’, Journal of Microbiology, Biotechnology, and Food Sciences.
Isriany, I 2016, ‘Aktivitas Antimikroba Hasil Fraksinasi Korte Kedondong pagar (Lannea coromandelica (Houtt.) Merr.)’
Joseph, et al 2013, An Investigation of The Phytochemistry And In- Vitro Cytotoxic Effect of The Aqueous Extract of Lannea Coromandelica. Bark, An International Journal Of Pharmaceutical Science Vol. 4, Issue 4, Supl 1. ISSN : 0976 : 7908
Kaur, R.., Jaiswal, M., & Jain, V. (2013). Protective Effec of Lannea coromandelica Houtt. Merril. Against Three Common Phatogens. Journal of Ayurveda and integrative medicine , 4/4, 224.
Kuntorini, E, M & Astuti, M, D 2010, ‘Penentuan Aktivitas Antioksidan Ekstrak Etanol Bulbus Bawang Dayak (Eleutherine americana Merr.)’, Jurnal Sains dan Terapan Kimia.
Manik, MA, Wahid, SMA, Islam, A, Pal, KT,
Ahmed 2013, ‘A Comparative Study
Rahayu, Sunarti, S, Diah, P, Suhardjono 2006, ‘Pemanfatan Tumbuhan Obat secara Tradisional oleh Masyarakat Lokal di Pulau Wawoni, Sulawesi Tenggara, Jurnal Biodiversitas Vol. 7 (3).
Rahim, A., Alam, G., Agustina, R., dan Rusydi, M., 2012, ‘Skrining toksisitas ekstrak herba bandotan (Ageratum conyzoides L.), Majalah Farmasi dan Farmakologi, hal 29-34.
Rao, VS., Eintein, JW, & Das, K 2014. Hepatoprotecctive And Antioxidant Activity Of Lannea coromandelica Linn. On Thioacetamide Induced Hepatotoxicity In Rats. International Letters Of Natural Science J. Vol.3 : 30-43. ISSN : 2300-9675
Ridho, AE, Rafika S, Sri w 2013, ‘Uji Aktivitas Antioksidan Ekstrak Metanol Buah Lakum dengan Metode Penangkapan radikal 2,2’-Difenil-1-Pikrilhidrazil’, Jurnal Studi Farmasi Fakultas Kedokteran, Universitas Tanjungpur.
Safriana 2014, ‘Pengaruh Pemberian Ekstrak Metanol Daun Kedondong Pagar (Lannea coromandelica (Houtt.) Merr) terhadap Penurunan Kadar Glukosa Darah pada Mencit’, Skripsi, Fakultas Kedokteran, Universitas Sumatera Utara.
Salamah, Nina, widyasari, Erlinda 2015, ‘Aktivitas antioksidan Ekstrak Metanol Daun Kalengkeng (Euphoria longan (L) Steud.) dengan Metode Penangkapan radikal 2,2’-Difenil-1-Pikrilhidrazil’, Jurnal Pharmaciana Universitas Ahmad Dahlan, Vol.5, No. 1, 2015; 25-34.
Sasidharan, N 2004, ‘Biodiversity documentation for KeralaPart 6, Flowering Plants, Kerala Forest Research Institute, Peechi, Kerala.
aktif Ketoprofen’. Jurnal, Jurusan Teknik Kimia, Fakultas Teknik Universitas Muhammadiyah Jakarta. Vol.2, No.2
Winarsi, H 2007, ‘Antioksidan Alami dan Radikal Bebas’, Yogyakarta : Kanisius, Deresan.
Prawirodiharjo, E 2014, ‘Uji Aktivitas Antioksidan dan Toksisitas Ekstrak Metanol 70% dan Ekstrak Air Kulit Batang Kedondong pagar (Lannea coromandelica)’, Skripsi, Jurusan Farmasi. Fakultas Kedokteran dan Ilmu Kesehatan UIN Syarif Hidayatullah, Jakarta
Priya, PD, Niranjana, CS, Anjali, SB 2014, ‘Sonneratia alba in J Smith’; 2012, A vital source og gamma linolenic acid (GLA), Asian J, Of Pharmaceutical and Clinical Research
Sayuti, K & R, Y 2015, ‘Antioksidan Alami & Sintetik’, Andalas University Press, Padang.
Sumarlin L.A, Muawanah A, Wardhani P, dan Masitoh 2014, ‘Aktivitas Antikanker dan Antioksidan Madu di Pasaran Lokal Indonesia’, Jurnal Ilmu Pertanian Indonesia, vol 19, pp. 136-144.
Tiwari, K, Kaur, M, Kaur, G & Kaur, H 2011, ‘Phytochemical Screening and Extraction’: A Review. Internationale Pharmaceutica Sciencia vol,1 :issue 1.
Tjitrosoepomo, G 2013, ‘Morfologi Tumbuhan’, Yogyakarta : Gajah Mada University Press
Tristantini, et al 2016, ‘ Pengujian Aktivitas Antioksidan Menggunakan Metode DPPH pada Daun Tanjung (Mimusops elengi L)’, Yogyakartab : Pengembangan Teknologi Kimia untuk Pengolaan Sumber DayaAlam Indonesia
Wahid Arif, ‘In Vitro Phytochemical and Biological Investigation of Plant Lanneacoromandelica (Family: Anacardiaceae)’, Thesis to Department of Pharmacy, East West University, Bangladesh.
Waris, R, Pratiwi, E, D, Najib, A 2016, ‘Radical Scavenging Activity of Leaf Extract of Edible Hibiscus (Abelmochus manihot (L.) Medik) Using 1,1 – Diphenyl – 2 – Picryl Hydrazil (DPPH), International Journal of Pharm Tech Research, Vol.9, No.6, pp 343-347.
Warono, D, Syamsudin, 2013, ‘Unjuk Kerja Spektrofotometer untuk Analisa Zat
