Analysis of UV protection activity of ethanol extract of cacao leaves (Theobroma cacao L.) based on Sun Protection Factor value.
Abstract
UV rays have a bad impact on the skin, but they can be minimized by using ingredients that are UV protective. Sunscreen can be obtained from natural ingredients, one of which is Cocoa Leaves (Theobroma cacao L.) which contains flavonoid and phenolic compounds. The aim of this research was to determine the UV protection activity of ethanol extract of cocoa leaves (Theobroma cacao L.) originating from the Malino, Jeneponto and Wajo areas based on the Sun Protection Factor value. Measurement of the Sun Protection Factor (SPF) value using the UV-Vis Spectrometry method was measured at wavelengths of 290, 295, 300, 305, 310, 315 and 320 nm. The results showed that the ethanol extract of cocoa leaves (Theobroma cacao L.) had UV protection activity for the Malino area at a concentration of 0.01% of 3.25 (minimum protection), a concentration of 0.03% of 10.34 (maximum protection). , 0.05% concentration of 17.10 (ultra protection). For the Jeneponto area, the 0.01% concentration is 3.88 (minimum protection), the 0.03% concentration is 12.3 (maximum protection), the 0.05% concentration is 20.18 (ultra protection) and the Wajo area has a value at a concentration of 0.01% it is 3.38 (minimum protection), a concentration of 0.03% is 10.77 (maximum protection), a concentration of 0.05% is 17.05 (ultra protection). Ethanol extract of cocoa leaves (Theobroma cacao L.) originating from Jeneponto has the greatest UV protection activity with a value of 20.18 with ultra protection ability.
References
[2] Pramiastuti, O. (2019). Penentuan Nilai Spf ( Sun Protection Factor) Ekstrak Dan Fraksi Daun Kecombrang (Etlingera Elatior) Secara in Vitro Menggunakan Metode Spektrofotometri. Parapemikir : Jurnal Ilmiah Farmasi, 8(1), 14.
[3] Utami, R. N., Rahmadani, A., & Ardana, M. (2017). Uji Aktivitas Tabir Surya Kombinasi Fraksi Etil Asetat Annona muricata Linn Folium, Artocarpus champeden Spreng Folium dan Plectranthus scutellaroides Folium Secara In Vitro. Proceeding of Mulawarman Pharmaceuticals Conferences, 6(November 2017), 155–166.
[4] Purwaningsih, S., Salamah, E., & Adnin, M. N. (2015). Photoprotective effect of sunscreen cream with addition of carrageenan and black mangrove fruit (Rhizopora mucronata Lamk.). Jurnal Ilmu Dan Teknologi Kelautan Tropis, 7(1), 1–14.
[5] Hasanah Sekolah Tinggi Ilmu Farmasi Bhakti Pertiwi Palembang Jl Ariodillah III No, M., Ilir Timur Palembang, A. I., & Selatan, S. (2016). Analisis Golongan Senyawa Kimia dan Uji Potensi Antioksidan dari Ekstrak Daun Cokelat (Theobroma cacao L.) Hasil Ekstraksi Maserasi. Jurnal Ilmiah Bakti Farmasi, 2, 43–48.
[6] Singh, N., Datta, S., Dey, A., Chowdhury, A. R., & Abraham, J. (2015). Antimicrobial activity and cytotoxicity
[7] Hasanah, S., Ahmad, I., & Rijai, L. (2015). Profil Tabir Surya Ekstrak dan Fraksi Daun Pidada Merah (Sonneratia caseolaris L.). Jurnal Sains Dan Kesehatan, 1(4).
[8] Ulfah, M., Mulyati, S., & Yunita, N. (2022). Standarisasi dan Aktivitas Tabir Surya Ekstrak Etanol Daun Kakao (Theobroma cacao L.). Jurnal Pharmascience, 9(1), 96-105.
[9] Istiawan, N. D., & Kastono, D. (2019). The effect of growing altitude on yield and oil quality of clove (Syzygium aromaticum (L.) Merr. & Perry.) in samigaluh sub-district, kulon progo. Jurnal Vegetalika, 8(1), 27–41.
[10] Setyo Utomo, D., Kristiani, E. B. E., & Mahardika, A. (2020). Pengaruh Lokasi Tumbuh Terhadap Kadar Flavonoid, Fenolik, Klorofil,Karotenoid Dan Aktivitas Antioksidan Pada Tumbuhan Pecut Kuda(Stachytarpheta Jamaicensis). Bioma, 22(2), 143–149.
[11] Pandey A, M. P. T. P. (2015). Constitutive espression of Arabidopsis MYB transcription factor, AtMYB11, in tobacco modulates flavonoid biosynthesis in favor o flavonol accumulation. Plant Cell Reports, 1115–1528.
[12] Maryam, S. M., Suhaenah, A., & Amrullah, N. F. (2020). Uji aktifitas Penghambatan Enzim α-Glukosidase ekstrak etanol biji buah alpukat sangrai (Persea americana Mill.) Secara In Vitro. Jurnal Ilmiah AsSyifaa, 12(1), 51–56.
[13] Tahir, M., Rahmawati, R., Maryam, S., Nurfauziah, P., & Nazhifah, N. (2022). Aktivitas senyawa flavanoid ekstrak etanol bunga kersen (Muntingia calabura L) sebagai tabir surya. As-Syifaa Jurnal Farmasi, 14(2), 97–104.
[14] Sutarna TH, Alatas F, Ratih H. Pengaruh Penambahan Vitamin C Sebagai Antioksidan Terhadap Nilai Sun Proctective Factor (SPF). Seminar Nasional Ilmu Pengetahuan dan Teknologi (IPTEK) Jenderal Ahmad Yani: Universitas Ahmad Yani, 2015
[15] Sineke, U. F., Edi., dan Sri, S. 2016. Penentuan Kandungan Fenolik dan Sun Protection Factor (SPF) dari Ekstrak Etanol dari Beberapa Tongkol Jagung (Zea mays L) Pharmacon. Jurnal Ilmiah Farmasi. 5 (1) ISSN 2302-2493
[16] Goh Khairudin K., Sukiran., Normah., Baharum. 2016. “Metabolite Profiling Reveals Temperature Effects On The Vocs And Flavonoids Of Different Plant Populations.” Plant Biol (Stuttg) 1: 130–39.
[17] Suradji, SI, Najib, A., & Ahmad, AR (2016). Studi Komparasi kadar flavonoid total pada bunga rosella merah (Hibiscus sabdariffa L.) asal Kabupaten Luwu Utara Provinsi Sulawesi Selatan dan Kabupaten Kediri Provinsi Jawa Timur. Jurnal Fitofarmaka Indonesia , 3 (2), 175-181.
[18] Kusbiantoro, D. Y. P. 2018. Pemanfaatan kandungan metabolit sekunder pada tanaman kunyit dalam mendukung peningkatan pendapatan masyarakat Utilization of secondary metabolite in the turmeric plant to increase community income. 17(1), 544–549
