Antioxidant activity Antioxidant activity of ethanol extract of bitti leaves (Vitex cofassus Reinw. ex. Blume) using the dpph free radical scavenging method
Keywords:
Bitti leaf extract (Vitex cofassus Reinw. Ex. Blume), antioxidant, DPPH, UV-Vis spectrophotometer.
Abstract
Antioxidants are compounds that can counteract free radicals in the body, reduce oxidation in cells and cell damage. The purpose of this study was to determine the antioxidant activity of ethanol extract of bitti leaves (Vitex cofassus Reinw. Ex. Blume with DPPH (2,2- diphenyl-1-picrylhydrazyl) scavenging method. The absorbance was measured by UV-Vis spectrophotometer at 510 nm wavelength. The results showed the presence of antioxidant activity with IC50 value of 56.59 μg/mL with strong antioxidant activity category, while quercetin as a comparator has very strong antioxidant activity with IC50 value of 5.93 μg/mL.
References
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[2] Anggarani, M.A., Ilmiah, M. and Mahfudhah, D.N. (2023) Literature Review of Antioxidant Activity of Several Types of Onions and Its Potensial as Health Supplements, pp. 1–9. doi:p-ISSN 2252-6951 e-ISSN 2502-6844.
[3] Susantiningsih, T. (2021) Biokimia Stres Oksidatif dan Prosedur Laboratorium (2 ed.) CV. Anugrah Utama Raharja
[4] Tandung, A.R., Manik, M.D. and Putri, T.Z.A.D. (2019) Hempaskan Nyamuk Aedes aegypti L. dengan Ekstrak Buah Bitti (Vitex cofassus), 7(2), pp. 50–51.
[5] Wulan and Yudistira, A. (2019) Uji Aktivitas Antioksidan dari Ekstrak Etanol Daun Mimosa pudica Linn. menggunakan Metode DPPH, 8(1), p. 109.
[6] Rani, A. and Sharma, A. (2013) ‘The genus vitex: A Review’, Pharmacognosy Reviews, 7(14), p.188.
[7] Syamsu, R.F. and Rachman, M.E. (2023) Uji Aktivitas Antioksidan Ekstrak Etanol Buah Tin (Ficus carica) dengan Metode DPPH dan FRAP, 15(1), pp. 81–84.
[8] Wakhidatul Kiromah, N.Z., Husein, S. and Rahayu, T.P. (2021) Uji Aktivitas Antioksidan Ekstrak Etanol Daun Ganitri (Elaeocarpus Ganitrus Roxb.) dengan Metode DPPH (2,2 Difenil-1-Pikrilhidazil) , 18(1), pp. 62–63.
[9] Risasti, S., Fitri and Oktiansyah, R. (2023) Uji Aktivitas Antioksidan Ekstrak Tanaman Obat dari Famili Zingiberaceae, pp. 1–7.
