PENENTUAN KADAR FENOLIK TOTAL PADA EKSTRAK ETANOL DAUN JERUJU (Acanthus ilicifolius L.) MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-VIS
Keywords:
Acanthus ilicifolius L.; ethanol extract; total phenolic content; UV-Vis spectrophotometry; Folin–Ciocalteu reagent.
Abstract
ABSTRACTAcanthus ilicifolius L., commonly known as Jeruju, is a mangrove shrub characterized by spiny branches and recognized for its potential medicinal properties. This study aimed to identify the presence of phenolic compounds and determine the total phenolic content (TPC) in the ethanolic extract of Jeruju leaves using UV-Visible spectrophotometry, with gallic acid as the calibration standard. The extraction was carried out by maceration using 96% ethanol. Qualitative phytochemical screening confirmed the presence of phenolic compounds, indicated by a color change to dark green. TPC was quantified using the Folin–Ciocalteu reagent at a wavelength of 759 nm. The results revealed that the ethanolic extract contained 64.815 mg gallic acid equivalents (GAE) per gram of extract. These findings demonstrate that Jeruju leaves are a rich source of phenolic compounds, supporting their potential use in pharmacological applications.
References
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[3] Bahar, Y., et al. (2021) ‘Penentuan Nilai Sun Protection Factor(SPF)Ekstrak Etanol Daun Jeruju (Acanthus IlicifoliusL.) secara In Vitro’, Indonesian Journal of Pharma Science, 3(2), pp. 91–96. https://online-journal.unja.ac.id/IJPS/article/view/15975
[4] Halal, A.H. and Asfar, A.M.I.T. dkk (2024) SABUN CUCI PIRING RAMAH LINGKUNGAN DARI LIMBAH KULIT PISANG DAN JERUK. Jakarta: PENERBIT KBM INDONESIA.
[5] Yumita, A. et al. (2023) ‘Penetapan Kadar Fenolik Total dan Kadar tanin Total Ekstrak Etanol 96% Daun Wujaya Kusuma(Epiphyllum oxypetalum (DC.) Haw.) Dengan Metode Spektrofotometri’, Jurnal WIYATA, 10(2), pp. 98–108. https://ojs.iik.ac.id/index.php/wiyata/article/view/554
[6] Tahir, M., Muflihunna, A. and Syafrianti. (2017) ‘Penentuan Kadar Fenolik Total Ekstrak Etanol Daun Nilam (Pogostemon cablin Benth.) dengan Metode Spektrofotometri UV-Vis’, Jurnal Fitofarmaka Indonesia, 4(1), pp. 215–218. https://jurnal.farmasi.umi.ac.id/index.php/fitofarmakaindo/article/view/231
[7] Fitriana, Asmaliani, I. and Ridwan, Y.A.A. (2024) ‘(Antibacterial Activity Test of Ethanol Extract from Dayak Onion Leaves (Eleutherine palmifolia (L.) Merr) Against Gastrointestinal Infection-Causing Bacteria Using the Agar Diffusion Method)’, As-Syifaa Jurnal Farmasi, 16(2), pp. 126–134. https://jurnal.farmasi.umi.ac.id/index.php/as-syifaa/article/view/1180
[8] Hartina, S., Aminah and Suhaenah, A. (2024) ‘Penetapan Kadar Fenolik dan Flavanoid Ekstrak Etanol Daun Sawo Manila (Manilkara zapota L.) Asal Kabupaten Wajo Secara Spektrofotometri UV-Vis’, Pharmaceutical Reports [Preprint]
[9] Hasnaeni, Wisdawati and Usman, S. (2019) ‘Pengaruh Metode Ekstraksi Terhadap Rendemen Dan Kadar Fenolik Ekstrak Tanaman Kayu Beta-Beta(Lunasia amara Blanco)’, Jurnal Farmasi Galenika, 5(2), pp. 175–182. doi:10.22487/j24428744.2019.v5.i2.13599.
[10] Salim, S.A. et al. (2020) ‘Review Artikel: Kelebihan dan Keterbatasan Pereaksi Folin-Cioucalteu dalam Penentuan Kadar Fenol Total pada Tanaman’, Farmaka, 18(1), pp. 46–57. https://jurnal.unpad.ac.id/farmaka/article/view/21909
[11] Suhaenah, A., Muflihunna, A. and Renhoran, A.A. (2024) ‘Penentuan Kadar Tanin Pada Ekstrak Etanol Daun Karet Kebo (Ficus elastica Roxb. ex Hornem) Dengan Menggunakan Metode Spektrofotometri UV-Vis’, Makassar Pharmaceutical Science Journal, 2(3), pp. 427–437. https://journal.farmasi.umi.ac.id/index.php/mpsj/article/view/281
[12] Afni, L. and et al. (2023) ‘Penetapan kadar fenolik total ekstrak kayu raru (Cotylelobium lanceolatum Craib) berdasarkan perbedaan konsentrasi etanol dengan metode spektrofotometri UV-Vis’, Journal of Pharmaceutical and Sciences, 6(4), pp. 1811–1818. https://repository.umnaw.ac.id/jspui/handle/123456789/3227
[13] Irawan, C. et al. (2025) ‘IDENTIFIKASI SECARA KIMIA GUGUS FUNGSI SENYAWA ORGANIK’. Yogyakarta: DEEPUBLISH.
[14] Candra, L.M.M., Andayani, Y. and Wirasisya, D.G. (2021) ‘Pengaruh Metode Ekstraksi Terhadap Kandungan Fenolik Total dan Flavanoid Total pada Ekstrak Etanol Buncis (Phaseolus vulgaris L.)’, J. Pijar MIPA, 16(3), pp. 397–405. https://jurnalfkip.unram.ac.id/index.php/JPM/article/view/2308
[15] Kusuma, A.E. and Aprileili, D.A. (2022) ‘Pengaruh Jumlah Pelarut Terhadap Rendemen Ekstrak Daun Katuk (Sauropus androgynus L. Merr)’, Jurnal Farmasi Sains dan Obat Tradisional, 1(2), pp. 125–135. https://ejournal.akfarimambonjol.ac.id/index.php/jfkes/article/view/22/17
[16] Winahyu, D.A., Retnaningsih, A. and Aprillia, M. (2019) ‘Penetapan Kadar Flavanoid Pada Kulit Batang Kayu Raru (Cotylelobium melanoxylon P) dengan Metode Spektrofotometri UV-Vis’, Jurnal Analisis Farmasi, 4(1), pp. 29–36. https://ejurnalmalahayati.ac.id/index.php/analis%20farmasi/article/view/1304
[17] Suryani, M.Y. et al. (2022) ‘Analisis Penentuan Kadar Besi (Fe) dalam Air Limbah Tambang Batu Bara Menggunakan Spektrofotometer UV-Vis’, Indonesian Journal of Laboratory, 5(1), pp. 7–15. https://jurnal.ugm.ac.id/ijl/article/view/72451
[18] Aryal, S. et al. (2019) ‘Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal’, Plants, 8(96), pp. 1–12. https://pubmed.ncbi.nlm.nih.gov/30978964/
