PENENTUAN KADAR FLAVONOID TOTAL EKSTRAK ETANOL DAUN JERUJU (Acanthus ilicifolius L.) MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-VIS
Keywords:
Flavonoids; Holy Mangrove Leaves; Acanthus ilicifolius L.; Ethanol Extract; UV-Vis Spectrophotometry
Abstract
ABSTRACT
Holy mangrove (Acanthus ilicifolius L.) is a medicinal plant rich in bioactive compounds, particularly flavonoids. Flavonoids exhibit significant antioxidant and anti-inflammatory properties. This study aimed to quantify the total flavonoid content (TFC) in the ethanolic extract of holy mangrove leaves using UV-Vis spectrophotometry. The leaves were dried, powdered, and extracted via maceration using 96% ethanol. Qualitative phytochemical screening confirmed the presence of flavonoids through characteristic color changes using magnesium powder, hydrochloric acid, and sodium hydroxide reagents. Quantitative analysis was performed using the aluminum chloride colorimetric method, with absorbance measured at 431 nm. Quercetin was used as the reference standard, and the TFC was expressed as quercetin equivalents (QE). The results revealed a high flavonoid content of 8.388 mg QE/g dry extract, underscoring the plant’s potential as a source of natural antioxidants and anti-inflammatory agents.
References
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[4] Abriyani, E. et al. (2024) ‘Literature Riview Artikel Identifikasi Kadar Flavonoid Total Dengan Metode Spektrofotometri Uv-Vis’, Jurnal Kesehatan Tambusai, 5(1), pp. 1575–1583.
[5] Handoyo Sahumena, M. et al. (2020) ‘Identifikasi Jamu Yang Beredar Di Kota Kendari Menggunakan Metode Spektrofotometri Uv-Vis’, Journal Syifa Sciences and Clinical Research, 2(2), pp. 65–72. Available at: https://doi.org/10.37311/jsscr.v2i2.6977.
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[7] Nur Fasya Milwan, A. (2023) ‘Aktivitas Antioksidan Ekstrak Daun Porang (Amorpophallus muelleri Blume) Secara Spektrofotomrtri UV-Vis’, Makassar Natural Product Journal, 1(3), pp. 2023–164. Available at: https://journal.farmasi.umi.ac.id/index.php/mnpj.
[8] Suhaenah, A. et al. (2023) ‘Skrining Fitokimis Dan Uji Aktivitas Antioksidan Fraksi Etil Asetat Daun Karet Kebo (Ficus elastica) Dengan Menggunakan Metode Perendaman Radikal Bebas DPPH (2,2-Diphenyl-1- Picrylhydrazil)’, As-Syifaa Jurnal Farmasi, 15(1), pp. 20–29. Available at: https://doi.org/10.56711/jifa.v15i1.935.
[9] Forestryana, D. et al. (2020) ‘Phytochemical Screenings And Thin Layer Chromatography Analysis Of Ethanol Extract Jeruju Leaf (Hydrolea Spinosa L.)’, Jurnal Ilmiah Farmako Bahari, pp. 113–124.
[10] Lindawati, N.Y. and Ma’ruf, S.H. (2020) ‘Penetapan Kadar Total Flavonoid Ekstrak Etanol Kacang Merah (Phaseolus vulgaris L.) Secara Spektrofotometri Visibel’, Jurnal Ilmiah Manuntung, 6(1), pp. 83–91. Available at: https://doi.org/10.51352/jim.v6i1.312.
[11] Lindawati, N.Y. and Ni’ma, A. (2022) ‘Analysis of Total Flavanoid Levels of Fennel Leaves (Foeniculum Vulgare) Ethanol Extract By Spectrophotometry Visibel’, Jurnal Farmasi Sains dan Praktis, 8(1), pp. 1–12. Available at: https://doi.org/10.31603/pharmacy.v8i1.4972.
[12] Elvira, E., Abidin, Z. and Razak, R. (2024) ‘Analisis Kandungan Flavonoid Ekstrak Etanol Daun Kenikir (Cosmos caudatus)’, Makassar Pharmaceutical Science Journal, 1(37), pp. 347–357. Available at: https://journal.farmasi.umi.ac.id/index.php/mpsj.
[13] Mahmud, N.F., Maryam, S. and Suhaenah, A. (2024) ‘Analisis Kadar Senyawa Flavonoid dari Ekstrak Etanol Daun Kakao (Theobroma cacao L.) Dengan Perbandingan Daerah Tempat Tumbuh’, Makassar Pharmaceutical Science Journal, 2(2), pp. 326–335. Available at: https://journal.farmasi.umi.ac.id/index.php/mpsj.
[14] Asworo, R.Y. and Widwiastuti, H. (2023) ‘Pengaruh Ukuran Serbuk Simplisia dan Waktu Maserasi terhadap Aktivitas Antioksidan Ekstrak Kulit Sirsak’, Indonesian Journal of Pharmaceutical Education, 3(2), pp. 256–263. Available at: https://doi.org/10.37311/ijpe.v3i2.19906.
[15] Adriana, U.H., Nofita and Selvi Marcelia (2024) ‘Uji aktivitas kombinasi ekstrak etanol daun kemangi (’, Jurnalmalahayat, 11(1), pp. 185–196.
[16] Sari, Y., Syahrul, S. and Iriani, D. (2021) ‘Skrining Fitokimia dan Aktivitas Antioksidan pada Kijing (Pylsbryoconcha Sp) dengan Pelarut Berbeda’, Jurnal Teknologi dan Industri Pertanian Indonesia, 13(1), pp. 16–20. Available at: https://doi.org/10.17969/jtipi.v13i1.18324.
[17] Apriliyani, S.A. et al. (2018) ‘Validation of UV-VIS Spectrophotometric Methods for Determination of Inulin Levels from Lesser Yam (Dioscorea esculenta L.)’, Jurnal Kimia Sains dan Aplikasi, 21(4), pp. 161–165. Available at: https://doi.org/10.14710/jksa.21.4.161-165.
[18] Pratama, M., Baits, M. and Ananda, S. (2023) ‘Penetapan Kadar Flavonoid Ekstrak Etanol Daun Tanaman Jongi (Dillenia serrata) Menggunakan Metode Spektrofotometri UV-Vis’, Makassar Pharmaceutical Science Journal, 1(3), pp. 224–232.
[19] Asmorowati, H. (2019) ‘Penetapan kadar flavonoid total buah alpukat biasa (Persea americana Mill.) dan alpukat mentega (Persea americana Mill.) dengan metode spektrofotometri UV-Vis’, Jurnal Ilmiah Farmasi, 15(2), pp. 51–63. Available at: https://doi.org/10.20885/jif.vol15.iss2.art1.
[20] Elsan, R. and Minarsih, T. (2022) ‘Analisis Sildenafil Sitrat dalam jamu kuat dengan metode Spektrofotometri UV-Vis’, Indonesian Journal of Pharmacy and Natural Product, 5(1), pp. 43–50. Available at: https://doi.org/10.35473/ijpnp.v5i1.1569.
