Antibacterial Activity Test of Ethanol Extract of Java Wood Leaves (Lannea coromandelica (Houtt.) Merr.) Against Bacteria That Cause Skin Infection Bacteria by Agar Diffusion Method

Aulia Putri Kayla; Fitriana Fitriana; Siska Nuryanti

Aulia Putri Kayla Universitas Muslim Indonesia
Fitriana Fitriana
Siska Nuryanti

Keywords: java wood leaves, antibacterial, skin infections, agar diffusion

Abstract

The purpose of this study was to determine the antibacterial activity of ethanol extract of Java wood leaves (Lannea coromandelica (Houtt.) Merr.) against bacteria that cause skin infections. The research method begins with an extraction process using the maceration method with 96% ethanol solvent and evaporated using a rotavapor. Java wood leaf ethanol extract was screened with a concentration of 0.1%; 0,5%; and 1% against bacteria Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Propionibacterium acnes. MIC and MBC tests were carried out and antibacterial activity tests were continued using the agar diffusion method with a concentration variation of 0.8%; 1,6%; 3,2%; 6,4%; and 12.8%. Screening test results of Staphylococcus aureus bacteria at 0.5% and Propionibacterium acnes bacteria at 0.1%. MIC value against Staphylococcus aureus bacteria at 0.2% and Propionibacterium acnes bacteria at 0.05%. While the MBC value against Staphylococcus aureus bacteria at 0.8% and Propionibacterium acnes bacteria at 1.6%. The results of testing the antibacterial activity of ethanol extract of Java wood leaves against Staphylococcus aureus and Propionibacterium acnes bacteria had the largest inhibitory zone diameter at a concentration of 12.8% of 11.32 mm and 12.83 mm respectively. Based on the results of research, ethanol extract of Java wood leaves has potential as an antibacterial.

References

[1] Suhara NA, Mauludiyah EN, Albab LU, Suhara NA, Maulana IT. Isolasi Fraksi Senyawa Aktif Antibakteri Staphylococcus epidermidis Dari Chlorella vulgaris B Sebagai Bahan Aktif antiseptik. J Ilm Farm Farmasyifa. 2020;3(1):18–25.
[2] Azizah M, Lingga LS, Rikmasari Y. Uji Aktivitas Antibakteri Kombinasi Ekstrak Etanol Daun Seledri (Apium graviolens L.) Dan Madu Hutan Terhadap Beberapa Bakteri Penyebab Penyakit Kulit. J Penelit Sains. 2020;22(1):37.
[3] Rusli R, Kosman R, Melinda P. PENELUSURAN FUNGI ENDOFIT PADA DAUN KOPASANDA (Chromolaena odorata L.) YANG BERPOTENSI SEBAGAI PENGHASIL ANTIBAKTERI TERHADAP BAKTERI PENYEBAB INFEKSI KULIT. J Ilm As-Syifaa. 2020;12(1):64–9.
[4] Semadhi PGM, Mahardika KIK, Megayanthi RS, Kirana, Ni Wayan Prabasiwi I Palaguna DGBP, Hendrayana MA. Uji aktivitas antibakteri ekstrak kulit batang tanaman kenanga ( cananga odorata ) terhadap bakteri penyebab infeksi kulit Staphylococcus aureus in vitro. Intisari Sains Medis. 2022;13(1):6–10.
[5] Purssell E. Antimicrobials. Understanding pharmacology in nursing practice. 2020:147-65.
[6] Puetri NR, Marlinda M, Yunsa B, Alegantina S, Sundari D. Uji Toksisitas Akut Ekstrak Etanol Daun Kayu Jawa (Lannea coromandelica (Houtt.) Merr.) pada Tikus Wistar. Media Penelit dan Pengemb Kesehat. 2021;31(4):357–62.
[7] Pagarra H. Analisis Fitokimia dan Pengaruh Ekstrak Etanol Daun Kayu Jawa ( Lannea coromandelica ) terhadap Pertumbuhan Bakteri Staphylococcus aureus Phytochemical Analysis and Effect of Ethanol Extract of Javanese Wood Leaves ( Lannea coromandelica ) on the Growth o. 2022;XI(2):135–43.
[8] Wendersteyt NV, Wewengkang DS, Abdullah SS. UJI AKTIVITAS ANTIMIKROBA DARI EKSTRAK DAN FRAKSI ASCIDIAN Herdmania momus DARI PERAIRAN PULAU BANGKA LIKUPANG TERHADAP PERTUMBUHAN MIKROBA Staphylococcus aureus, Salmonella typhimurium DAN Candida albicans. Pharmacon. 2021;10(1):706.
[9] Suhendar U, Utami NF, Sutanto D, Nurdayanty SM. PENGARUH BERBAGAI METODE EKSTRAKSI PADA PENENTUAN KADAR FLAVONOID EKSTRAK ETANOL DAUN ILER (Plectranthus scutellarioides). FITOFARMAKA J Ilm Farm. 2020;10(1):76–83.
[10] Maryam S, Juniasti S, Kosman R. Uji Aktivitas Antibakteri Ekstrak Etanol Buah Belimbing Wuluh. As-Syifaa J Farm. 2015;7(1):60–9.
[11] Utomo SB, Fujiyanti M, Lestari WP, Mulyani S. Antibacterial Activity Test of the C-4-methoxyphenylcalix[4]resorcinarene Compound Modified by Hexadecyltrimethylammonium-Bromide against Staphylococcus aureus and Escherichia coli Bacteria. JKPK (Jurnal Kim dan Pendidik Kim. 2018;3(3):201.
[12] Purwanitiningsih E, Lestari D. Uji Aktivitas Antibakteri Ekstrak Daun Cocor Bebek (Kalanchoe Pinnata (Lam)) Terhadap Pertumbuhan Bakteri Salmonella typhi Dengan Metode Kirby Bauer. J Ilm Kesehat. 2020;12(2):142–8.
[13] Susanty S, Bachmid F. Perbandingan metode ekstraksi maserasi dan refluks terhadap kadar fenolik dari ekstrak tongkol jagung (Zea mays L.). Jurnal Konversi. 2016 Oct 10;5(2):87-92.